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KMID : 0604219950020010141
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Korean Journal Investigative Dermatology
1995 Volume.2 No. 1 p.141 ~ p.150
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The Effects of Supernatant and Cell Extract of Cultured Human Melanocyte on the Growth of Cultured Human Kcratinocyte
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Abstract
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The main function of melanocyte is known to protect the skin from hazardous sunlight. Some investigators have claimed lately that melanocyte in also related to the immunological role in the epidermis and may be involve the growth of
keratinocytes.
We therefore investigated the possibility that soluble factors or cytokines released from melanocytes regulate the proliferation of keratinocytes. To evaluate the effects of supernatant (MS) and extract(ME) of cultured human melanocyte on the
morphological changes and proliferation of cultured human keratinocytes, the supernatant was added to keratinocyte growth medium (KGM) to be 50% (MS-I) and the cell extract was added to KGM as 100μg protein/l ml KGM(ME-I). In addition, to
evaluate
the
effects of UVB. Cultured melanocytes were irradiated at 15mJ/cm2 of UVB once. Thensupernatant (MS-II) and cell extract (ME-KK) of cultured human melanocytes were collected and added to KGM. In order to evaluate relationship between concentrations
of MS
and ME and morphologic changes and proliferation of cultured human keratinocytes, different concentrations of MS and ME were added to KGM.
On morphological changes. We observed more enlarged cells and vacuolated cells in MS groups and ME groups than in the control. These findings showed doe-dependent manner. The proliferation of keratinocytes was decreased in MS groups and ME
groups,
and
the decrease apperared inversed pattern to concentration of MS and ME(P<0.05). The effects of UVB irradiation on morphologic changes and proliferation of keratinocytes were not significant.
The sum of these results suggested that MS and ME induced morphological changes and early terminal differentiation and decreased proliferation of cultured human keratinocytes. So we propose that melanocytes release certain factors or cytokines
which
could modulate the proliferation and differentiation of keratinocytes.
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KEYWORD
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